It comes five days after their disappointing 1-1 draw with Morecambe on Easter Monday, where the Blues conceded a controversial 93rd-minute equaliser against the struggling Shrimps.
The former Lincoln boss made three changes for the trip to the Mazuma Stadium, with Joe Morrell missing out completely after a failed fitness test.
However, the Welsh international is back in contention and could be one of three changes to the starting line-up against Gillingham.
Pompey will be without injured Denver Hume, who remains sidelined with a back injury, while Reeco Hackett (knee) and Kieron Freeman will continue to sit out the rest of the season.
With Saturday marking the penultimate game at PO4 this season, here’s how we predict the Blues’ squad to shape up against Neil Harris’ side.
However, the Welsh international is back in contention and could be one of three changes to the starting line-up against Gillingham.
![Despite being the Blues' top scorer this season, Cowley was unimpressed with his performance at the Mazuma Stadium and is could find himself on the bench against Gillingham.](https://www.portsmouth.co.uk/webimg/b25lY21zOmZkODE5ODRjLTg0OWItNDBlMC1iNGZhLWRmYzU2Y2U5ZjMwZjphODI3ZGQ0Zi1iMmE3LTRiZjQtOTFkNC02ZjE1MDRhYjM4ODQ=.jpg?crop=3:2&trim=&width=640)
17. SUB 6: Marcus Harness
Despite being the Blues' top scorer this season, Cowley was unimpressed with his performance at the Mazuma Stadium and is could find himself on the bench against Gillingham.Photo: JPIMedia
![He hasn't played a single minute since his underwhelming performance against Cheltenham a fortnight ago and Cowley admits his move to Pompey hasn't worked out.](https://www.portsmouth.co.uk/webimg/b25lY21zOjQ3NWJlMTI0LTBmNTctNGQ5NS04ZGI1LTFiNzBmOTJjZWQ4ODpkODNhZmJmNS05MzY1LTQ0OWEtYWU5ZS00ZmVkZGE3NmE2YTY=.jpg?crop=3:2&trim=&width=640)
18. SUB 7: Tyler Walker
He hasn't played a single minute since his underwhelming performance against Cheltenham a fortnight ago and Cowley admits his move to Pompey hasn't worked out.Photo: The News