It was a contest already filled with fire and fury, with Harry Redknapp and Jim Smith making their much-anticipated return to Fratton Park following their departure in November 2004.
It also saw the Blues’ Division One title winner Nigel Quashie start for the Saints just months after the midfielder swapped PO4 for St Mary’s in the January transfer window.
With a capacity crowd at Fratton Park, Alain Perrin’s men got off to the best start when Yakubu netted from the spot after just four minutes. The lead was then doubled by captain Arjan De Zeeuw, who headed home Patrik Berger’s 17th-minute free kick.
Although Henri Camara got a goal back, a spectacular double from Lomana LuaLua sealed the dominant 4-1 victory, while also confirming Pompey’s top flight status.
Meanwhile, Southampton would go onto finish the season in the bottom three, with West Brom beating the Blues on the final day to help relegate their bitter rivals.
With the demolition derby now 20 years old and with the prospect of another two meetings between the two sides, we’ve delved into the archives to find the best photos from the 4-1 triumph.
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13. Pompey 4-1 Southampton: 20 years on
Fierce challenge on Diomansy Kamara from Danny Higginbottom. | Getty Images
14. Pompey 4-1 Southampton: 20 years on
Antti Niemi spills the ball to LuaLua, who converted impressively to net Pompey's third. | Getty Images
15. Pompey 4-1 Southampton: 20 years on
Stone gets past Quashie - no doubt prompting plenty of cheers from the Fratton faithful. | JONATHAN BRADY
16. Pompey 4-1 Southampton: 20 years on
No one can forget LuaLua's iconic backflips! | Getty Images